<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2025 Workiva-->
<!--r:19581f7a-925f-4c1f-ac05-fd6ccdd896cf,g:3955fb61-0ae2-4749-b8bf-d20be7a02309-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:roleRef roleURI="http://www.vistagen.com/role/Cover" xlink:type="simple" xlink:href="vtgn-20250114.xsd#Cover"/>
  <link:presentationLink xlink:role="http://www.vistagen.com/role/Cover" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CoverAbstract"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_20f89838-3290-4919-adf7-408dfd0fce59" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentType"/>
    <link:presentationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_DocumentType_20f89838-3290-4919-adf7-408dfd0fce59" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_cc389b08-4014-4509-853a-7bd8a26e314b" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentPeriodEndDate"/>
    <link:presentationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_DocumentPeriodEndDate_cc389b08-4014-4509-853a-7bd8a26e314b" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_ec815706-19ea-4919-8e20-03a8875be03d" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityRegistrantName"/>
    <link:presentationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_EntityRegistrantName_ec815706-19ea-4919-8e20-03a8875be03d" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_3a52efc2-1885-4b97-a0aa-662c3d45563c" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:presentationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_EntityIncorporationStateCountryCode_3a52efc2-1885-4b97-a0aa-662c3d45563c" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_431a0cea-d215-4a50-b4f1-48d88a87550e" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityFileNumber"/>
    <link:presentationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_EntityFileNumber_431a0cea-d215-4a50-b4f1-48d88a87550e" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_e152381b-8709-49bc-8a0b-bb911093eb19" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:presentationArc order="6" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_EntityTaxIdentificationNumber_e152381b-8709-49bc-8a0b-bb911093eb19" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_941ee6e7-afb8-4ac6-b208-8f3dcb8a008a" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressAddressLine1"/>
    <link:presentationArc order="7" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_EntityAddressAddressLine1_941ee6e7-afb8-4ac6-b208-8f3dcb8a008a" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_8d1e228e-0c63-4fc7-90f7-a9722892ac4d" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressCityOrTown"/>
    <link:presentationArc order="8" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_EntityAddressCityOrTown_8d1e228e-0c63-4fc7-90f7-a9722892ac4d" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_53462f7c-94d8-4f17-b3eb-6a6f11b74dab" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressStateOrProvince"/>
    <link:presentationArc order="9" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_EntityAddressStateOrProvince_53462f7c-94d8-4f17-b3eb-6a6f11b74dab" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_56b037b0-b4d2-44d6-bbfd-d17a0d94bfec" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressPostalZipCode"/>
    <link:presentationArc order="10" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_EntityAddressPostalZipCode_56b037b0-b4d2-44d6-bbfd-d17a0d94bfec" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_d0c9a145-0237-4e6f-8d3d-6d8768f6e3e4" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CityAreaCode"/>
    <link:presentationArc order="11" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_CityAreaCode_d0c9a145-0237-4e6f-8d3d-6d8768f6e3e4" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_f94b672c-b95d-4d51-b35e-8927c6ed4e94" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_LocalPhoneNumber"/>
    <link:presentationArc order="12" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_LocalPhoneNumber_f94b672c-b95d-4d51-b35e-8927c6ed4e94" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_WrittenCommunications_17915221-ed1c-4a93-bae8-bcf4556f7eb7" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_WrittenCommunications"/>
    <link:presentationArc order="13" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_WrittenCommunications_17915221-ed1c-4a93-bae8-bcf4556f7eb7" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SolicitingMaterial_c03a7546-6493-4d54-a1a2-cf6300708f4d" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_SolicitingMaterial"/>
    <link:presentationArc order="14" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_SolicitingMaterial_c03a7546-6493-4d54-a1a2-cf6300708f4d" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_PreCommencementTenderOffer_8cf82a6e-810b-4b0d-82db-7d60dc3dc047" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_PreCommencementTenderOffer"/>
    <link:presentationArc order="15" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_PreCommencementTenderOffer_8cf82a6e-810b-4b0d-82db-7d60dc3dc047" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_PreCommencementIssuerTenderOffer_b817e382-3443-455b-8e35-d2fcdf737dc4" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_PreCommencementIssuerTenderOffer"/>
    <link:presentationArc order="16" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_PreCommencementIssuerTenderOffer_b817e382-3443-455b-8e35-d2fcdf737dc4" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_9af57cfa-1d1a-4b12-b593-bfb2dc8004e8" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_Security12bTitle"/>
    <link:presentationArc order="17" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_Security12bTitle_9af57cfa-1d1a-4b12-b593-bfb2dc8004e8" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_fe1a93d8-0f42-4ec5-893e-82dc8ad86e49" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_TradingSymbol"/>
    <link:presentationArc order="18" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_TradingSymbol_fe1a93d8-0f42-4ec5-893e-82dc8ad86e49" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_d834f911-1b57-4e91-8171-c1f11a0c1032" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_SecurityExchangeName"/>
    <link:presentationArc order="19" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_SecurityExchangeName_d834f911-1b57-4e91-8171-c1f11a0c1032" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_e4a22405-e92b-45c0-812c-9253f1983b57" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:presentationArc order="20" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_EntityEmergingGrowthCompany_e4a22405-e92b-45c0-812c-9253f1983b57" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_c31d8e17-aca7-4f13-a00c-1c66c4adc94b" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_AmendmentFlag"/>
    <link:presentationArc order="21" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_AmendmentFlag_c31d8e17-aca7-4f13-a00c-1c66c4adc94b" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_b3ff2fb3-973b-489d-823a-13b61cf78b4e" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCentralIndexKey"/>
    <link:presentationArc order="22" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_c6f685ab-a31c-4d7f-9af3-056a4f56fa63" xlink:to="loc_dei_EntityCentralIndexKey_b3ff2fb3-973b-489d-823a-13b61cf78b4e" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
  </link:presentationLink>
</link:linkbase>
