<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2026 Workiva-->
<!--r:019fd725-ad1b-7630-b343-0ad854a689b0,g:fdfea0ae-b109-4277-a01b-3c08b93514ed-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:roleRef roleURI="http://www.vistagen.com/role/Cover" xlink:type="simple" xlink:href="vtgn-20260806.xsd#Cover"/>
  <link:presentationLink xlink:role="http://www.vistagen.com/role/Cover" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CoverAbstract"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_019fd725-ad1b-7ae3-8d14-484ae6a88021" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentType"/>
    <link:presentationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_DocumentType_019fd725-ad1b-7ae3-8d14-484ae6a88021" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_019fd725-ad1b-7a25-9ac7-7fc6aa40a9b9" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentPeriodEndDate"/>
    <link:presentationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_DocumentPeriodEndDate_019fd725-ad1b-7a25-9ac7-7fc6aa40a9b9" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_019fd725-ad1b-7339-8296-1704b1277045" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityRegistrantName"/>
    <link:presentationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_EntityRegistrantName_019fd725-ad1b-7339-8296-1704b1277045" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_019fd725-ad1b-7729-acee-173b98b39463" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:presentationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_EntityIncorporationStateCountryCode_019fd725-ad1b-7729-acee-173b98b39463" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_019fd725-ad1b-75db-8267-84d1b679b6f8" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityFileNumber"/>
    <link:presentationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_EntityFileNumber_019fd725-ad1b-75db-8267-84d1b679b6f8" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_019fd725-ad1b-75dc-81fc-b2df709f5ba1" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:presentationArc order="6" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_EntityTaxIdentificationNumber_019fd725-ad1b-75dc-81fc-b2df709f5ba1" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_019fd725-ad1b-79c3-91bf-5e17a9df0c46" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressAddressLine1"/>
    <link:presentationArc order="7" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_EntityAddressAddressLine1_019fd725-ad1b-79c3-91bf-5e17a9df0c46" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_019fd725-ad1b-78dd-9048-e4e0a542d5e7" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressCityOrTown"/>
    <link:presentationArc order="8" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_EntityAddressCityOrTown_019fd725-ad1b-78dd-9048-e4e0a542d5e7" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_019fd725-ad1b-7008-8a21-194394a08413" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressStateOrProvince"/>
    <link:presentationArc order="9" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_EntityAddressStateOrProvince_019fd725-ad1b-7008-8a21-194394a08413" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_019fd725-ad1b-75f6-93bc-7fe460bc146b" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressPostalZipCode"/>
    <link:presentationArc order="10" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_EntityAddressPostalZipCode_019fd725-ad1b-75f6-93bc-7fe460bc146b" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_019fd725-ad1b-7982-b1f8-d00e505b0969" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CityAreaCode"/>
    <link:presentationArc order="11" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_CityAreaCode_019fd725-ad1b-7982-b1f8-d00e505b0969" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_019fd725-ad1b-7150-88f2-b366aac04439" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_LocalPhoneNumber"/>
    <link:presentationArc order="12" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_LocalPhoneNumber_019fd725-ad1b-7150-88f2-b366aac04439" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_WrittenCommunications_019fd725-ad1b-761a-be45-20e34d3f2864" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_WrittenCommunications"/>
    <link:presentationArc order="13" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_WrittenCommunications_019fd725-ad1b-761a-be45-20e34d3f2864" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SolicitingMaterial_019fd725-ad1b-784c-b6b6-2e952b5e3f60" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_SolicitingMaterial"/>
    <link:presentationArc order="14" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_SolicitingMaterial_019fd725-ad1b-784c-b6b6-2e952b5e3f60" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_PreCommencementTenderOffer_019fd725-ad1b-7ea9-97a9-f6a0cfa0d529" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_PreCommencementTenderOffer"/>
    <link:presentationArc order="15" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_PreCommencementTenderOffer_019fd725-ad1b-7ea9-97a9-f6a0cfa0d529" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_PreCommencementIssuerTenderOffer_019fd725-ad1b-7363-b7d6-b37bf4fa5226" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_PreCommencementIssuerTenderOffer"/>
    <link:presentationArc order="16" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_PreCommencementIssuerTenderOffer_019fd725-ad1b-7363-b7d6-b37bf4fa5226" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_019fd725-ad1b-73fc-8eca-2d568f1306f8" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_Security12bTitle"/>
    <link:presentationArc order="17" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_Security12bTitle_019fd725-ad1b-73fc-8eca-2d568f1306f8" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_019fd725-ad1b-7a32-ba90-220444bebad6" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_TradingSymbol"/>
    <link:presentationArc order="18" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_TradingSymbol_019fd725-ad1b-7a32-ba90-220444bebad6" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_019fd725-ad1b-7b67-9f1f-e8c96142a7f1" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_SecurityExchangeName"/>
    <link:presentationArc order="19" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_SecurityExchangeName_019fd725-ad1b-7b67-9f1f-e8c96142a7f1" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_019fd725-ad1b-7c1e-860a-e5736f77ef2d" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:presentationArc order="20" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_EntityEmergingGrowthCompany_019fd725-ad1b-7c1e-860a-e5736f77ef2d" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_019fd725-ad1b-7aec-8a9a-ba2649e7005f" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_AmendmentFlag"/>
    <link:presentationArc order="21" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_AmendmentFlag_019fd725-ad1b-7aec-8a9a-ba2649e7005f" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_019fd725-ad1b-7447-a54c-4d3af0a1c787" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCentralIndexKey"/>
    <link:presentationArc order="22" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019fd725-ad1b-7c69-9527-9981c8465c14" xlink:to="loc_dei_EntityCentralIndexKey_019fd725-ad1b-7447-a54c-4d3af0a1c787" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
  </link:presentationLink>
</link:linkbase>
