<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2026 Workiva-->
<!--r:019cf787-22ae-7c11-a201-95a84cf15e48,g:7629c0d8-e9ca-40a8-b9cb-240b7c0558eb-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:roleRef roleURI="http://www.vistagen.com/role/Cover" xlink:type="simple" xlink:href="vtgn-20260316.xsd#Cover"/>
  <link:presentationLink xlink:role="http://www.vistagen.com/role/Cover" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CoverAbstract"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_019cf787-22ae-7f9d-8b03-ca313b68f07e" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentType"/>
    <link:presentationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_DocumentType_019cf787-22ae-7f9d-8b03-ca313b68f07e" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_019cf787-22ae-731a-ae0d-1d08f7f149d3" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentPeriodEndDate"/>
    <link:presentationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_DocumentPeriodEndDate_019cf787-22ae-731a-ae0d-1d08f7f149d3" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_019cf787-22ae-799f-a20f-9a060dfeb4fc" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityRegistrantName"/>
    <link:presentationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_EntityRegistrantName_019cf787-22ae-799f-a20f-9a060dfeb4fc" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_019cf787-22ae-7e9b-b4db-f0e452b9c1a2" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:presentationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_EntityIncorporationStateCountryCode_019cf787-22ae-7e9b-b4db-f0e452b9c1a2" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_019cf787-22ae-7707-9fbd-213389769627" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityFileNumber"/>
    <link:presentationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_EntityFileNumber_019cf787-22ae-7707-9fbd-213389769627" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_019cf787-22ae-7797-b229-a1a1bbf33318" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:presentationArc order="6" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_EntityTaxIdentificationNumber_019cf787-22ae-7797-b229-a1a1bbf33318" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_019cf787-22ae-7709-8910-c5de41d88b61" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressAddressLine1"/>
    <link:presentationArc order="7" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_EntityAddressAddressLine1_019cf787-22ae-7709-8910-c5de41d88b61" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_019cf787-22ae-79f5-814c-efcbb7fa33d1" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressCityOrTown"/>
    <link:presentationArc order="8" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_EntityAddressCityOrTown_019cf787-22ae-79f5-814c-efcbb7fa33d1" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_019cf787-22ae-7498-a6a0-1ebe4758069e" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressStateOrProvince"/>
    <link:presentationArc order="9" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_EntityAddressStateOrProvince_019cf787-22ae-7498-a6a0-1ebe4758069e" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_019cf787-22ae-74d5-af8c-39920d9a8cb3" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressPostalZipCode"/>
    <link:presentationArc order="10" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_EntityAddressPostalZipCode_019cf787-22ae-74d5-af8c-39920d9a8cb3" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_019cf787-22ae-78b9-9316-5a51ef37281a" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CityAreaCode"/>
    <link:presentationArc order="11" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_CityAreaCode_019cf787-22ae-78b9-9316-5a51ef37281a" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_019cf787-22ae-790a-8190-a211e69e1047" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_LocalPhoneNumber"/>
    <link:presentationArc order="12" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_LocalPhoneNumber_019cf787-22ae-790a-8190-a211e69e1047" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_WrittenCommunications_019cf787-22ae-7d86-971e-066c07fae472" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_WrittenCommunications"/>
    <link:presentationArc order="13" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_WrittenCommunications_019cf787-22ae-7d86-971e-066c07fae472" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SolicitingMaterial_019cf787-22ae-7f6d-98b2-2b34e607fbec" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_SolicitingMaterial"/>
    <link:presentationArc order="14" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_SolicitingMaterial_019cf787-22ae-7f6d-98b2-2b34e607fbec" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_PreCommencementTenderOffer_019cf787-22ae-76a5-9eab-612d4b49b0ab" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_PreCommencementTenderOffer"/>
    <link:presentationArc order="15" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_PreCommencementTenderOffer_019cf787-22ae-76a5-9eab-612d4b49b0ab" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_PreCommencementIssuerTenderOffer_019cf787-22ae-7ca9-9112-cef8721478b1" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_PreCommencementIssuerTenderOffer"/>
    <link:presentationArc order="16" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_PreCommencementIssuerTenderOffer_019cf787-22ae-7ca9-9112-cef8721478b1" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_019cf787-22ae-7aad-8e16-2816908ac0f9" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_Security12bTitle"/>
    <link:presentationArc order="17" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_Security12bTitle_019cf787-22ae-7aad-8e16-2816908ac0f9" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_019cf787-22ae-764b-acd7-33c47b02ea13" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_TradingSymbol"/>
    <link:presentationArc order="18" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_TradingSymbol_019cf787-22ae-764b-acd7-33c47b02ea13" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_019cf787-22ae-74f2-8833-6e3c100aecd9" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_SecurityExchangeName"/>
    <link:presentationArc order="19" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_SecurityExchangeName_019cf787-22ae-74f2-8833-6e3c100aecd9" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_019cf787-22ae-772c-aaeb-6d871d69fc11" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:presentationArc order="20" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_EntityEmergingGrowthCompany_019cf787-22ae-772c-aaeb-6d871d69fc11" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_019cf787-22ae-7833-8b1d-4cb3b3adfff1" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_AmendmentFlag"/>
    <link:presentationArc order="21" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_AmendmentFlag_019cf787-22ae-7833-8b1d-4cb3b3adfff1" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_019cf787-22ae-7340-9fdd-05ce6e9db704" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCentralIndexKey"/>
    <link:presentationArc order="22" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_019cf787-22ae-7148-adff-5bedd3af425f" xlink:to="loc_dei_EntityCentralIndexKey_019cf787-22ae-7340-9fdd-05ce6e9db704" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
  </link:presentationLink>
</link:linkbase>
