<?xml version="1.0" encoding="US-ASCII" standalone="no"?>
    <!-- Field: Doc-Info; Name: Generator; Value: GoFiler Complete; Version: 4.15a -->
    <!-- Field: Doc-Info; Name: VendorURI; Value: http://www.novaworks.co -->
    <!-- Field: Doc-Info; Name: Source; Value: Vistagen 03312017 10K.xfr; Date: 2017%2D06%2D28T20:04:17Z -->
    <!-- Field: Doc-Info; Name: Status; Value: 0x00000000 -->
<link:linkbase xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xbrli="http://www.xbrl.org/2003/instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
    <link:roleRef roleURI="http://vistagen.com/role/DocumentAndEntityInformation" xlink:href="vtgn-20170331.xsd#DocumentAndEntityInformation" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/ConsolidatedBalanceSheets" xlink:href="vtgn-20170331.xsd#ConsolidatedBalanceSheets" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/ConsolidatedBalanceSheetsParenthetical" xlink:href="vtgn-20170331.xsd#ConsolidatedBalanceSheetsParenthetical" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/ConsolidatedStatementsOfOperationsAndComprehensiveLoss" xlink:href="vtgn-20170331.xsd#ConsolidatedStatementsOfOperationsAndComprehensiveLoss" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/ConsolidatedStatementsOfCashFlows" xlink:href="vtgn-20170331.xsd#ConsolidatedStatementsOfCashFlows" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/ConsolidatedStatementsOfStockholdersDeficit" xlink:href="vtgn-20170331.xsd#ConsolidatedStatementsOfStockholdersDeficit" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/DescriptionOfBusiness" xlink:href="vtgn-20170331.xsd#DescriptionOfBusiness" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/BasisOfPresentation" xlink:href="vtgn-20170331.xsd#BasisOfPresentation" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/SummaryOfSignificantAccountingPolicies" xlink:href="vtgn-20170331.xsd#SummaryOfSignificantAccountingPolicies" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/FairValueMeasurements" xlink:href="vtgn-20170331.xsd#FairValueMeasurements" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/PrepaidExpensesAndOtherCurrentAssets" xlink:href="vtgn-20170331.xsd#PrepaidExpensesAndOtherCurrentAssets" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/PropertyAndEquipment" xlink:href="vtgn-20170331.xsd#PropertyAndEquipment" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/AccruedExpenses" xlink:href="vtgn-20170331.xsd#AccruedExpenses" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/NotesPayable" xlink:href="vtgn-20170331.xsd#NotesPayable" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/CapitalStock" xlink:href="vtgn-20170331.xsd#CapitalStock" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/ResearchAndDevelopmentExpenses" xlink:href="vtgn-20170331.xsd#ResearchAndDevelopmentExpenses" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/IncomeTaxes" xlink:href="vtgn-20170331.xsd#IncomeTaxes" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/LicensingAndCollaborativeAgreements" xlink:href="vtgn-20170331.xsd#LicensingAndCollaborativeAgreements" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/StockOptionPlansAnd401kPlan" xlink:href="vtgn-20170331.xsd#StockOptionPlansAnd401kPlan" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/RelatedPartyTransactions" xlink:href="vtgn-20170331.xsd#RelatedPartyTransactions" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/CommitmentsContingenciesGuaranteesAndIndemnifications" xlink:href="vtgn-20170331.xsd#CommitmentsContingenciesGuaranteesAndIndemnifications" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/SubsequentEvents" xlink:href="vtgn-20170331.xsd#SubsequentEvents" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/SupplementalFinancialInformation" xlink:href="vtgn-20170331.xsd#SupplementalFinancialInformation" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/SummaryOfSignificantAccountingPoliciesPolicies" xlink:href="vtgn-20170331.xsd#SummaryOfSignificantAccountingPoliciesPolicies" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/SummaryOfSignificantAccountingPoliciesTables" xlink:href="vtgn-20170331.xsd#SummaryOfSignificantAccountingPoliciesTables" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/PrepaidExpensesAndOtherCurrentAssetsTables" xlink:href="vtgn-20170331.xsd#PrepaidExpensesAndOtherCurrentAssetsTables" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/PropertyAndEquipmentTables" xlink:href="vtgn-20170331.xsd#PropertyAndEquipmentTables" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/AccruedExpensesTables" xlink:href="vtgn-20170331.xsd#AccruedExpensesTables" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/ConvertiblePromissoryNotesAndOtherNotesPayableTables" xlink:href="vtgn-20170331.xsd#ConvertiblePromissoryNotesAndOtherNotesPayableTables" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/CapitalStockTables" xlink:href="vtgn-20170331.xsd#CapitalStockTables" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/IncomeTaxesTables" xlink:href="vtgn-20170331.xsd#IncomeTaxesTables" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/StockOptionPlansAnd401kPlanTables" xlink:href="vtgn-20170331.xsd#StockOptionPlansAnd401kPlanTables" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/CommitmentsContingenciesGuaranteesAndIndemnificationsTables" xlink:href="vtgn-20170331.xsd#CommitmentsContingenciesGuaranteesAndIndemnificationsTables" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/SupplementalFinancialInformationTables" xlink:href="vtgn-20170331.xsd#SupplementalFinancialInformationTables" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/BasisOfPresentationDetailsNarrative" xlink:href="vtgn-20170331.xsd#BasisOfPresentationDetailsNarrative" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/SummaryOfSignificantAccountingPoliciesDetails" xlink:href="vtgn-20170331.xsd#SummaryOfSignificantAccountingPoliciesDetails" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/SummaryOfSignificantAccountingPoliciesDetails1" xlink:href="vtgn-20170331.xsd#SummaryOfSignificantAccountingPoliciesDetails1" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/SummaryOfSignificantAccountingPoliciesDetailsNarrative" xlink:href="vtgn-20170331.xsd#SummaryOfSignificantAccountingPoliciesDetailsNarrative" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/PrepaidExpensesAndOtherCurrentAssetsDetails" xlink:href="vtgn-20170331.xsd#PrepaidExpensesAndOtherCurrentAssetsDetails" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/PropertyAndEquipmentDetails" xlink:href="vtgn-20170331.xsd#PropertyAndEquipmentDetails" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/AccruedExpensesDetails" xlink:href="vtgn-20170331.xsd#AccruedExpensesDetails" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/ConvertiblePromissoryNotesAndOtherNotesPayableDetails" xlink:href="vtgn-20170331.xsd#ConvertiblePromissoryNotesAndOtherNotesPayableDetails" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/CapitalStockDetails" xlink:href="vtgn-20170331.xsd#CapitalStockDetails" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/CapitalStockDetails1" xlink:href="vtgn-20170331.xsd#CapitalStockDetails1" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/CapitalStockDetails2" xlink:href="vtgn-20170331.xsd#CapitalStockDetails2" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/CapitalStockDetails3" xlink:href="vtgn-20170331.xsd#CapitalStockDetails3" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/CapitalStockDetails4" xlink:href="vtgn-20170331.xsd#CapitalStockDetails4" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/CapitalStockDetails5" xlink:href="vtgn-20170331.xsd#CapitalStockDetails5" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/CapitalStockDetails6" xlink:href="vtgn-20170331.xsd#CapitalStockDetails6" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/ResearchAndDevelopmentExpensesDetailsNarrative" xlink:href="vtgn-20170331.xsd#ResearchAndDevelopmentExpensesDetailsNarrative" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/IncomeTaxesDetails" xlink:href="vtgn-20170331.xsd#IncomeTaxesDetails" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/IncomeTaxesDetails1" xlink:href="vtgn-20170331.xsd#IncomeTaxesDetails1" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/IncomeTaxesDetails2" xlink:href="vtgn-20170331.xsd#IncomeTaxesDetails2" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/IncomeTaxesDetailsNarrative" xlink:href="vtgn-20170331.xsd#IncomeTaxesDetailsNarrative" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/LicensingSublicensingAndCollaborativeAgreementsDetailsNarrative" xlink:href="vtgn-20170331.xsd#LicensingSublicensingAndCollaborativeAgreementsDetailsNarrative" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/StockOptionPlansAnd401kPlanDetails" xlink:href="vtgn-20170331.xsd#StockOptionPlansAnd401kPlanDetails" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/StockOptionPlansAnd401kPlanDetails1" xlink:href="vtgn-20170331.xsd#StockOptionPlansAnd401kPlanDetails1" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/StockOptionPlansAnd401kPlanDetails2" xlink:href="vtgn-20170331.xsd#StockOptionPlansAnd401kPlanDetails2" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/StockOptionPlansAnd401kPlanDetails3" xlink:href="vtgn-20170331.xsd#StockOptionPlansAnd401kPlanDetails3" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/StockOptionPlansAnd401kPlanDetailsNarrative" xlink:href="vtgn-20170331.xsd#StockOptionPlansAnd401kPlanDetailsNarrative" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/RelatedPartyTransactionsDetailsNarrative" xlink:href="vtgn-20170331.xsd#RelatedPartyTransactionsDetailsNarrative" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/CommitmentsContingenciesGuaranteesAndIndemnificationsDetails" xlink:href="vtgn-20170331.xsd#CommitmentsContingenciesGuaranteesAndIndemnificationsDetails" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/CommitmentsContingenciesGuaranteesAndIndemnificationsDetails1" xlink:href="vtgn-20170331.xsd#CommitmentsContingenciesGuaranteesAndIndemnificationsDetails1" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/CommitmentsContingenciesGuaranteesAndIndemnificationsDetails2" xlink:href="vtgn-20170331.xsd#CommitmentsContingenciesGuaranteesAndIndemnificationsDetails2" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/CommitmentsContingenciesGuaranteesAndIndemnificationsDetailsNarrative" xlink:href="vtgn-20170331.xsd#CommitmentsContingenciesGuaranteesAndIndemnificationsDetailsNarrative" xlink:type="simple" />
    <link:roleRef roleURI="http://vistagen.com/role/SupplementalFinancialInformationDetails" xlink:href="vtgn-20170331.xsd#SupplementalFinancialInformationDetails" xlink:type="simple" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/DocumentAndEntityInformation" xlink:title="00000001 - Document - Document and Entity Information" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/ConsolidatedBalanceSheets" xlink:title="00000002 - Statement - CONSOLIDATED BALANCE SHEETS">
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AssetsCurrent" xlink:label="loc_us-gaapAssetsCurrent" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue" xlink:label="loc_us-gaapCashAndCashEquivalentsAtCarryingValue" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_us-gaapCashAndCashEquivalentsAtCarryingValue" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent" xlink:label="loc_us-gaapPrepaidExpenseAndOtherAssetsCurrent" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_us-gaapPrepaidExpenseAndOtherAssetsCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_Assets" xlink:label="loc_us-gaapAssets" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssets" xlink:to="loc_us-gaapAssetsCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PropertyPlantAndEquipmentNet" xlink:label="loc_us-gaapPropertyPlantAndEquipmentNet" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssets" xlink:to="loc_us-gaapPropertyPlantAndEquipmentNet" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="vtgn-20170331.xsd#vtgn_SecurityDepositsAndOtherAssets" xlink:label="loc_vtgnSecurityDepositsAndOtherAssets" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssets" xlink:to="loc_vtgnSecurityDepositsAndOtherAssets" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LiabilitiesCurrent" xlink:label="loc_us-gaapLiabilitiesCurrent" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccountsPayableCurrent" xlink:label="loc_us-gaapAccountsPayableCurrent" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesCurrent" xlink:to="loc_us-gaapAccountsPayableCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccruedLiabilitiesCurrent" xlink:label="loc_us-gaapAccruedLiabilitiesCurrent" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesCurrent" xlink:to="loc_us-gaapAccruedLiabilitiesCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CapitalLeaseObligationsCurrent" xlink:label="loc_us-gaapCapitalLeaseObligationsCurrent" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesCurrent" xlink:to="loc_us-gaapCapitalLeaseObligationsCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NotesPayableCurrent" xlink:label="loc_us-gaapNotesPayableCurrent" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesCurrent" xlink:to="loc_us-gaapNotesPayableCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LiabilitiesNoncurrent" xlink:label="loc_us-gaapLiabilitiesNoncurrent" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LongTermNotesPayable" xlink:label="loc_us-gaapLongTermNotesPayable" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesNoncurrent" xlink:to="loc_us-gaapLongTermNotesPayable" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DeferredRentCreditNoncurrent" xlink:label="loc_us-gaapDeferredRentCreditNoncurrent" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesNoncurrent" xlink:to="loc_us-gaapDeferredRentCreditNoncurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CapitalLeaseObligationsNoncurrent" xlink:label="loc_us-gaapCapitalLeaseObligationsNoncurrent" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesNoncurrent" xlink:to="loc_us-gaapCapitalLeaseObligationsNoncurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="vtgn-20170331.xsd#vtgn_AccruedDividendsNoncurrent" xlink:label="loc_vtgnAccruedDividendsNoncurrent" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesNoncurrent" xlink:to="loc_vtgnAccruedDividendsNoncurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_Liabilities" xlink:label="loc_us-gaapLiabilities" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilities" xlink:to="loc_us-gaapLiabilitiesCurrent" xlink:type="arc" weight="1" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilities" xlink:to="loc_us-gaapLiabilitiesNoncurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_StockholdersEquity" xlink:label="loc_us-gaapStockholdersEquity" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PreferredStockValue" xlink:label="loc_us-gaapPreferredStockValue" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquity" xlink:to="loc_us-gaapPreferredStockValue" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CommonStockValue" xlink:label="loc_us-gaapCommonStockValue" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquity" xlink:to="loc_us-gaapCommonStockValue" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AdditionalPaidInCapital" xlink:label="loc_us-gaapAdditionalPaidInCapital" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquity" xlink:to="loc_us-gaapAdditionalPaidInCapital" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_TreasuryStockValue" xlink:label="loc_us-gaapTreasuryStockValue" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquity" xlink:to="loc_us-gaapTreasuryStockValue" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RetainedEarningsAccumulatedDeficit" xlink:label="loc_us-gaapRetainedEarningsAccumulatedDeficit" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquity" xlink:to="loc_us-gaapRetainedEarningsAccumulatedDeficit" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LiabilitiesAndStockholdersEquity" xlink:label="loc_us-gaapLiabilitiesAndStockholdersEquity" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesAndStockholdersEquity" xlink:to="loc_us-gaapLiabilities" xlink:type="arc" weight="1" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesAndStockholdersEquity" xlink:to="loc_us-gaapStockholdersEquity" xlink:type="arc" weight="1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/ConsolidatedBalanceSheetsParenthetical" xlink:title="00000003 - Statement - CONSOLIDATED BALANCE SHEETS (Parenthetical)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/ConsolidatedStatementsOfOperationsAndComprehensiveLoss" xlink:title="00000004 - Statement - CONSOLIDATED STATEMENTS OF OPERATIONS AND COMPREHENSIVE LOSS">
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_Revenues" xlink:label="loc_us-gaapRevenues" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_LicensesRevenue" xlink:label="loc_us-gaapLicensesRevenue" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapRevenues" xlink:to="loc_us-gaapLicensesRevenue" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_OperatingExpenses" xlink:label="loc_us-gaapOperatingExpenses" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ResearchAndDevelopmentExpense" xlink:label="loc_us-gaapResearchAndDevelopmentExpense" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingExpenses" xlink:to="loc_us-gaapResearchAndDevelopmentExpense" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_GeneralAndAdministrativeExpense" xlink:label="loc_us-gaapGeneralAndAdministrativeExpense" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingExpenses" xlink:to="loc_us-gaapGeneralAndAdministrativeExpense" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_OperatingIncomeLoss" xlink:label="loc_us-gaapOperatingIncomeLoss" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingIncomeLoss" xlink:to="loc_us-gaapOperatingExpenses" xlink:type="arc" weight="-1" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingIncomeLoss" xlink:to="loc_us-gaapRevenues" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest" xlink:label="loc_us-gaapIncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest" xlink:to="loc_us-gaapOperatingIncomeLoss" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_InterestExpense" xlink:label="loc_us-gaapInterestExpense" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest" xlink:to="loc_us-gaapInterestExpense" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="vtgn-20170331.xsd#vtgn_ChangeInWarrantLiability" xlink:label="loc_vtgnChangeInWarrantLiability" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest" xlink:to="loc_vtgnChangeInWarrantLiability" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt" xlink:label="loc_us-gaapGainsLossesOnExtinguishmentOfDebt" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest" xlink:to="loc_us-gaapGainsLossesOnExtinguishmentOfDebt" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_OtherNonoperatingIncomeExpense" xlink:label="loc_us-gaapOtherNonoperatingIncomeExpense" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest" xlink:to="loc_us-gaapOtherNonoperatingIncomeExpense" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetIncomeLoss" xlink:label="loc_us-gaapNetIncomeLoss" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetIncomeLoss" xlink:to="loc_us-gaapIncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncomeTaxExpenseBenefit" xlink:label="loc_us-gaapIncomeTaxExpenseBenefit" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetIncomeLoss" xlink:to="loc_us-gaapIncomeTaxExpenseBenefit" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic" xlink:label="loc_us-gaapNetIncomeLossAvailableToCommonStockholdersBasic" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetIncomeLossAvailableToCommonStockholdersBasic" xlink:to="loc_us-gaapNetIncomeLoss" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="vtgn-20170331.xsd#vtgn_AccruedDividendOnSeriesBPreferredStock" xlink:label="loc_vtgnAccruedDividendOnSeriesBPreferredStock" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetIncomeLossAvailableToCommonStockholdersBasic" xlink:to="loc_vtgnAccruedDividendOnSeriesBPreferredStock" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="vtgn-20170331.xsd#vtgn_DeemedDividendOnSeriesBPreferredUnits" xlink:label="loc_vtgnDeemedDividendOnSeriesBPreferredUnits" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetIncomeLossAvailableToCommonStockholdersBasic" xlink:to="loc_vtgnDeemedDividendOnSeriesBPreferredUnits" xlink:type="arc" weight="-1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/ConsolidatedStatementsOfCashFlows" xlink:title="00000005 - Statement - CONSOLIDATED STATEMENTS OF CASH FLOWS">
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:label="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetIncomeLoss" xlink:label="loc_us-gaapNetIncomeLoss" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:to="loc_us-gaapNetIncomeLoss" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DepreciationDepletionAndAmortization" xlink:label="loc_us-gaapDepreciationDepletionAndAmortization" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:to="loc_us-gaapDepreciationDepletionAndAmortization" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AmortizationOfFinancingCostsAndDiscounts" xlink:label="loc_us-gaapAmortizationOfFinancingCostsAndDiscounts" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:to="loc_us-gaapAmortizationOfFinancingCostsAndDiscounts" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="vtgn-20170331.xsd#vtgn_ChangeInWarrantLiability" xlink:label="loc_vtgnChangeInWarrantLiability" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:to="loc_vtgnChangeInWarrantLiability" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="vtgn-20170331.xsd#vtgn_ExpenseRelatedToModificationOfWarrantsExchangeOfWarrantsForSeriesCPreferredAndCommonStock" xlink:label="loc_vtgnExpenseRelatedToModificationOfWarrantsExchangeOfWarrantsForSeriesCPreferredAndCommonStock" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:to="loc_vtgnExpenseRelatedToModificationOfWarrantsExchangeOfWarrantsForSeriesCPreferredAndCommonStock" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AmortizationOfDeferredLeasingFees" xlink:label="loc_us-gaapAmortizationOfDeferredLeasingFees" />
      <link:calculationArc order="6" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:to="loc_us-gaapAmortizationOfDeferredLeasingFees" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="vtgn-20170331.xsd#vtgn_FairValueOfCommonStockGrantedForServices" xlink:label="loc_vtgnFairValueOfCommonStockGrantedForServices" />
      <link:calculationArc order="7" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:to="loc_vtgnFairValueOfCommonStockGrantedForServices" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="vtgn-20170331.xsd#vtgn_FairValueOfSeriesBPreferredStockGrantedForServices" xlink:label="loc_vtgnFairValueOfSeriesBPreferredStockGrantedForServices" />
      <link:calculationArc order="8" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:to="loc_vtgnFairValueOfSeriesBPreferredStockGrantedForServices" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AdjustmentOfWarrantsGrantedForServices" xlink:label="loc_us-gaapAdjustmentOfWarrantsGrantedForServices" />
      <link:calculationArc order="9" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:to="loc_us-gaapAdjustmentOfWarrantsGrantedForServices" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ForeignCurrencyTransactionGainLossBeforeTax" xlink:label="loc_us-gaapForeignCurrencyTransactionGainLossBeforeTax" />
      <link:calculationArc order="10" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:to="loc_us-gaapForeignCurrencyTransactionGainLossBeforeTax" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt" xlink:label="loc_us-gaapGainsLossesOnExtinguishmentOfDebt" />
      <link:calculationArc order="11" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:to="loc_us-gaapGainsLossesOnExtinguishmentOfDebt" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_GainLossOnSaleOfPropertyPlantEquipment" xlink:label="loc_us-gaapGainLossOnSaleOfPropertyPlantEquipment" />
      <link:calculationArc order="12" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:to="loc_us-gaapGainLossOnSaleOfPropertyPlantEquipment" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets" xlink:label="loc_us-gaapIncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets" />
      <link:calculationArc order="13" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:to="loc_us-gaapIncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_IncreaseDecreaseInAccountsPayableAndAccruedLiabilities" xlink:label="loc_us-gaapIncreaseDecreaseInAccountsPayableAndAccruedLiabilities" />
      <link:calculationArc order="14" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:to="loc_us-gaapIncreaseDecreaseInAccountsPayableAndAccruedLiabilities" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AllocatedShareBasedCompensationExpense" xlink:label="loc_us-gaapAllocatedShareBasedCompensationExpense" />
      <link:calculationArc order="15" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:to="loc_us-gaapAllocatedShareBasedCompensationExpense" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities" xlink:label="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment" xlink:label="loc_us-gaapPaymentsToAcquirePropertyPlantAndEquipment" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:to="loc_us-gaapPaymentsToAcquirePropertyPlantAndEquipment" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivitiesContinuingOperations" xlink:label="loc_us-gaapNetCashProvidedByUsedInFinancingActivitiesContinuingOperations" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ProceedsFromIssuanceOfCommonStock" xlink:label="loc_us-gaapProceedsFromIssuanceOfCommonStock" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInFinancingActivitiesContinuingOperations" xlink:to="loc_us-gaapProceedsFromIssuanceOfCommonStock" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_ProceedsFromIssuanceOfPreferredStockAndPreferenceStock" xlink:label="loc_us-gaapProceedsFromIssuanceOfPreferredStockAndPreferenceStock" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInFinancingActivitiesContinuingOperations" xlink:to="loc_us-gaapProceedsFromIssuanceOfPreferredStockAndPreferenceStock" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RepaymentsOfLongTermCapitalLeaseObligations" xlink:label="loc_us-gaapRepaymentsOfLongTermCapitalLeaseObligations" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInFinancingActivitiesContinuingOperations" xlink:to="loc_us-gaapRepaymentsOfLongTermCapitalLeaseObligations" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_RepaymentsOfNotesPayable" xlink:label="loc_us-gaapRepaymentsOfNotesPayable" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInFinancingActivitiesContinuingOperations" xlink:to="loc_us-gaapRepaymentsOfNotesPayable" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CashAndCashEquivalentsPeriodIncreaseDecrease" xlink:label="loc_us-gaapCashAndCashEquivalentsPeriodIncreaseDecrease" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapCashAndCashEquivalentsPeriodIncreaseDecrease" xlink:to="loc_us-gaapNetCashProvidedByUsedInOperatingActivitiesContinuingOperations" xlink:type="arc" weight="1" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapCashAndCashEquivalentsPeriodIncreaseDecrease" xlink:to="loc_us-gaapNetCashProvidedByUsedInFinancingActivitiesContinuingOperations" xlink:type="arc" weight="1" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapCashAndCashEquivalentsPeriodIncreaseDecrease" xlink:to="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:type="arc" weight="1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/ConsolidatedStatementsOfStockholdersDeficit" xlink:title="00000006 - Statement - CONSOLIDATED STATEMENTS OF STOCKHOLDERS' DEFICIT" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/DescriptionOfBusiness" xlink:title="00000007 - Disclosure - Description of Business" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/BasisOfPresentation" xlink:title="00000008 - Disclosure - Basis of Presentation and Going Concern" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/SummaryOfSignificantAccountingPolicies" xlink:title="00000009 - Disclosure - Summary of Significant Accounting Policies" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/FairValueMeasurements" xlink:title="00000010 - Disclosure - Fair Value Measurements" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/PrepaidExpensesAndOtherCurrentAssets" xlink:title="00000011 - Disclosure - Prepaid Expenses and Other Current Assets" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/PropertyAndEquipment" xlink:title="00000012 - Disclosure - Property and Equipment" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/AccruedExpenses" xlink:title="00000013 - Disclosure - Accrued Expenses" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/NotesPayable" xlink:title="00000014 - Disclosure - Notes Payable" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/CapitalStock" xlink:title="00000015 - Disclosure - Capital Stock" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/ResearchAndDevelopmentExpenses" xlink:title="00000016 - Disclosure - Research and Development Expenses" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/IncomeTaxes" xlink:title="00000017 - Disclosure - Income Taxes" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/LicensingAndCollaborativeAgreements" xlink:title="00000018 - Disclosure - Licensing, Sublicensing and Collaborative Agreements" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/StockOptionPlansAnd401kPlan" xlink:title="00000019 - Disclosure - Stock Option Plans and 401(k) Plan" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/RelatedPartyTransactions" xlink:title="00000020 - Disclosure - Related Party Transactions" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/CommitmentsContingenciesGuaranteesAndIndemnifications" xlink:title="00000021 - Disclosure - Commitments, Contingencies, Guarantees and Indemnifications" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/SubsequentEvents" xlink:title="00000022 - Disclosure - Subsequent Events" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/SupplementalFinancialInformation" xlink:title="00000023 - Disclosure - Supplemental Financial Information" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/SummaryOfSignificantAccountingPoliciesPolicies" xlink:title="00000024 - Disclosure - Summary of Significant Accounting Policies (Policies)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/SummaryOfSignificantAccountingPoliciesTables" xlink:title="00000025 - Disclosure - Summary of Significant Accounting Policies (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/PrepaidExpensesAndOtherCurrentAssetsTables" xlink:title="00000026 - Disclosure - Prepaid Expenses and Other Current Assets (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/PropertyAndEquipmentTables" xlink:title="00000027 - Disclosure - Property and Equipment (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/AccruedExpensesTables" xlink:title="00000028 - Disclosure - Accrued Expenses (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/ConvertiblePromissoryNotesAndOtherNotesPayableTables" xlink:title="00000029 - Disclosure - Notes Payable (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/CapitalStockTables" xlink:title="00000030 - Disclosure - Capital Stock (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/IncomeTaxesTables" xlink:title="00000031 - Disclosure - Income Taxes (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/StockOptionPlansAnd401kPlanTables" xlink:title="00000032 - Disclosure - Stock Option Plans and 401(k) Plan (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/CommitmentsContingenciesGuaranteesAndIndemnificationsTables" xlink:title="00000033 - Disclosure - Commitments, Contingencies, Guarantees and Indemnifications (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/SupplementalFinancialInformationTables" xlink:title="00000034 - Disclosure - Supplemental Financial Information (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/BasisOfPresentationDetailsNarrative" xlink:title="00000035 - Disclosure - Basis of Presentation (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/SummaryOfSignificantAccountingPoliciesDetails" xlink:title="00000036 - Disclosure - Summary of Significant Accounting Policies (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/SummaryOfSignificantAccountingPoliciesDetails1" xlink:title="00000037 - Disclosure - Summary of Significant Accounting Policies (Details 1)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/SummaryOfSignificantAccountingPoliciesDetailsNarrative" xlink:title="00000038 - Disclosure - Summary of Significant Accounting Policies (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/PrepaidExpensesAndOtherCurrentAssetsDetails" xlink:title="00000039 - Disclosure - Prepaid Expenses and Other Current Assets (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/PropertyAndEquipmentDetails" xlink:title="00000040 - Disclosure - Property and Equipment (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/AccruedExpensesDetails" xlink:title="00000041 - Disclosure - Accrued Expenses (Details)">
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccruedLiabilitiesCurrent" xlink:label="loc_us-gaapAccruedLiabilitiesCurrent" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_AccruedProfessionalFeesCurrent" xlink:label="loc_us-gaapAccruedProfessionalFeesCurrent" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAccruedLiabilitiesCurrent" xlink:to="loc_us-gaapAccruedProfessionalFeesCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="vtgn-20170331.xsd#vtgn_AccruedDevelopmentExpenses" xlink:label="loc_vtgnAccruedDevelopmentExpenses" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAccruedLiabilitiesCurrent" xlink:to="loc_vtgnAccruedDevelopmentExpenses" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="vtgn-20170331.xsd#vtgn_AccruedCompensation" xlink:label="loc_vtgnAccruedCompensation" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAccruedLiabilitiesCurrent" xlink:to="loc_vtgnAccruedCompensation" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_OtherAccruedLiabilitiesCurrent" xlink:label="loc_us-gaapOtherAccruedLiabilitiesCurrent" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAccruedLiabilitiesCurrent" xlink:to="loc_us-gaapOtherAccruedLiabilitiesCurrent" xlink:type="arc" weight="1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/ConvertiblePromissoryNotesAndOtherNotesPayableDetails" xlink:title="00000042 - Disclosure - Convertible Promissory Notes and Other Notes Payable (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/CapitalStockDetails" xlink:title="00000043 - Disclosure - Capital Stock (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/CapitalStockDetails1" xlink:title="00000044 - Disclosure - Capital Stock (Details 1)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/CapitalStockDetails2" xlink:title="00000045 - Disclosure - Capital Stock (Details 2)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/CapitalStockDetails3" xlink:title="00000046 - Disclosure - Capital Stock (Details 3)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/CapitalStockDetails4" xlink:title="00000047 - Disclosure - Capital Stock (Details 4)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/CapitalStockDetails5" xlink:title="00000048 - Disclosure - Capital Stock (Details 5)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/CapitalStockDetails6" xlink:title="00000049 - Disclosure - Capital Stock (Details 6)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/ResearchAndDevelopmentExpensesDetailsNarrative" xlink:title="00000050 - Disclosure - Research and Development Expenses (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/IncomeTaxesDetails" xlink:title="00000051 - Disclosure - Income Taxes (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/IncomeTaxesDetails1" xlink:title="00000052 - Disclosure - Income Taxes (Details 1)">
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DeferredTaxAssetsGross" xlink:label="loc_us-gaapDeferredTaxAssetsGross" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DeferredTaxAssetsOperatingLossCarryforwards" xlink:label="loc_us-gaapDeferredTaxAssetsOperatingLossCarryforwards" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapDeferredTaxAssetsGross" xlink:to="loc_us-gaapDeferredTaxAssetsOperatingLossCarryforwards" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="vtgn-20170331.xsd#vtgn_DeferredTaxAssetsDifferencesInFixedAssets" xlink:label="loc_vtgnDeferredTaxAssetsDifferencesInFixedAssets" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapDeferredTaxAssetsGross" xlink:to="loc_vtgnDeferredTaxAssetsDifferencesInFixedAssets" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DeferredTaxAssetsTaxDeferredExpenseReservesAndAccruals" xlink:label="loc_us-gaapDeferredTaxAssetsTaxDeferredExpenseReservesAndAccruals" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapDeferredTaxAssetsGross" xlink:to="loc_us-gaapDeferredTaxAssetsTaxDeferredExpenseReservesAndAccruals" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DeferredTaxAssetsNet" xlink:label="loc_us-gaapDeferredTaxAssetsNet" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapDeferredTaxAssetsNet" xlink:to="loc_us-gaapDeferredTaxAssetsGross" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_DeferredTaxAssetsValuationAllowance" xlink:label="loc_us-gaapDeferredTaxAssetsValuationAllowance" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapDeferredTaxAssetsNet" xlink:to="loc_us-gaapDeferredTaxAssetsValuationAllowance" xlink:type="arc" weight="-1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/IncomeTaxesDetails2" xlink:title="00000053 - Disclosure - Income Taxes (Details 2)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/IncomeTaxesDetailsNarrative" xlink:title="00000054 - Disclosure - Income Taxes (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/LicensingSublicensingAndCollaborativeAgreementsDetailsNarrative" xlink:title="00000055 - Disclosure - Licensing, Sublicensing and Collaborative Agreements (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/StockOptionPlansAnd401kPlanDetails" xlink:title="00000056 - Disclosure - Stock Option Plans and 401(k) Plan (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/StockOptionPlansAnd401kPlanDetails1" xlink:title="00000057 - Disclosure - Stock Option Plans and 401(k) Plan (Details 1)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/StockOptionPlansAnd401kPlanDetails2" xlink:title="00000058 - Disclosure - Stock Option Plans and 401(k) Plan (Details 2)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/StockOptionPlansAnd401kPlanDetails3" xlink:title="00000059 - Disclosure - Stock Option Plans and 401(k) Plan (Details 3)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/StockOptionPlansAnd401kPlanDetailsNarrative" xlink:title="00000060 - Disclosure - Stock Option Plans and 401(k) Plan (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/RelatedPartyTransactionsDetailsNarrative" xlink:title="00000061 - Disclosure - Related Party Transactions (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/CommitmentsContingenciesGuaranteesAndIndemnificationsDetails" xlink:title="00000062 - Disclosure - Commitments, Contingencies, Guarantees and Indemnifications (Details)">
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CapitalLeasesBalanceSheetAssetsByMajorClassNet" xlink:label="loc_us-gaapCapitalLeasesBalanceSheetAssetsByMajorClassNet" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CapitalLeasedAssetsGross" xlink:label="loc_us-gaapCapitalLeasedAssetsGross" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapCapitalLeasesBalanceSheetAssetsByMajorClassNet" xlink:to="loc_us-gaapCapitalLeasedAssetsGross" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="http://xbrl.fasb.org/us-gaap/2016/elts/us-gaap-2016-01-31.xsd#us-gaap_CapitalLeasesLesseeBalanceSheetAssetsByMajorClassAccumulatedDeprecation" xlink:label="loc_us-gaapCapitalLeasesLesseeBalanceSheetAssetsByMajorClassAccumulatedDeprecation" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapCapitalLeasesBalanceSheetAssetsByMajorClassNet" xlink:to="loc_us-gaapCapitalLeasesLesseeBalanceSheetAssetsByMajorClassAccumulatedDeprecation" xlink:type="arc" weight="-1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/CommitmentsContingenciesGuaranteesAndIndemnificationsDetails1" xlink:title="00000063 - Disclosure - Commitments, Contingencies, Guarantees and Indemnifications (Details 1)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/CommitmentsContingenciesGuaranteesAndIndemnificationsDetails2" xlink:title="00000064 - Disclosure - Commitments, Contingencies, Guarantees and Indemnifications (Details 2)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/CommitmentsContingenciesGuaranteesAndIndemnificationsDetailsNarrative" xlink:title="00000065 - Disclosure - Commitments, Contingencies, Guarantees and Indemnifications (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://vistagen.com/role/SupplementalFinancialInformationDetails" xlink:title="00000066 - Disclosure - Supplemental Financial Information (Details)" />
</link:linkbase>
