<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2025 Workiva-->
<!--r:2f6cee45-dda0-4a57-be88-50f531990e87,g:3ee4eff9-5f31-4f52-b8d4-21d21f272529-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:roleRef roleURI="http://www.vistagen.com/role/Cover" xlink:type="simple" xlink:href="vtgn-20250623.xsd#Cover"/>
  <link:presentationLink xlink:role="http://www.vistagen.com/role/Cover" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CoverAbstract"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_36dc40d4-b92d-40fa-a918-ca7cc1ba767c" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentType"/>
    <link:presentationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_DocumentType_36dc40d4-b92d-40fa-a918-ca7cc1ba767c" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_6b47e266-8fa4-4a6f-bb0c-aee5184e41f9" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentPeriodEndDate"/>
    <link:presentationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_DocumentPeriodEndDate_6b47e266-8fa4-4a6f-bb0c-aee5184e41f9" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_bcf52830-5a40-474e-baf6-1063c99afa69" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityRegistrantName"/>
    <link:presentationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_EntityRegistrantName_bcf52830-5a40-474e-baf6-1063c99afa69" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_315fa53d-7c1c-4a5e-938b-18f6550913ca" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:presentationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_EntityIncorporationStateCountryCode_315fa53d-7c1c-4a5e-938b-18f6550913ca" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_4f177842-747a-4955-a3a4-d83924de28a2" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityFileNumber"/>
    <link:presentationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_EntityFileNumber_4f177842-747a-4955-a3a4-d83924de28a2" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_f0d02278-35e8-4d33-af14-e431d6b35ce5" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:presentationArc order="6" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_EntityTaxIdentificationNumber_f0d02278-35e8-4d33-af14-e431d6b35ce5" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_c393c458-1875-4ca9-ba3c-50044d5eae40" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressAddressLine1"/>
    <link:presentationArc order="7" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_EntityAddressAddressLine1_c393c458-1875-4ca9-ba3c-50044d5eae40" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_37c01c96-53e5-4822-a857-e9336233ebd6" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressCityOrTown"/>
    <link:presentationArc order="8" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_EntityAddressCityOrTown_37c01c96-53e5-4822-a857-e9336233ebd6" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_686038d3-8d8e-49a9-82de-3177c59e296d" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressStateOrProvince"/>
    <link:presentationArc order="9" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_EntityAddressStateOrProvince_686038d3-8d8e-49a9-82de-3177c59e296d" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_1c2412bd-450e-48bc-a133-708b74c90e99" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressPostalZipCode"/>
    <link:presentationArc order="10" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_EntityAddressPostalZipCode_1c2412bd-450e-48bc-a133-708b74c90e99" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_eea49743-8b72-438c-a457-c9daf9c09b63" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CityAreaCode"/>
    <link:presentationArc order="11" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_CityAreaCode_eea49743-8b72-438c-a457-c9daf9c09b63" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_0f7fa103-f9d2-48ad-8260-6ed10e5d32a9" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_LocalPhoneNumber"/>
    <link:presentationArc order="12" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_LocalPhoneNumber_0f7fa103-f9d2-48ad-8260-6ed10e5d32a9" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_WrittenCommunications_db3b5227-060f-4506-8200-477ecf666d4e" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_WrittenCommunications"/>
    <link:presentationArc order="13" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_WrittenCommunications_db3b5227-060f-4506-8200-477ecf666d4e" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SolicitingMaterial_6284f4a9-b84c-4077-9612-a4203f0c38b3" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_SolicitingMaterial"/>
    <link:presentationArc order="14" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_SolicitingMaterial_6284f4a9-b84c-4077-9612-a4203f0c38b3" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_PreCommencementTenderOffer_2853f231-a3b2-4df8-9293-5191ff494e3f" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_PreCommencementTenderOffer"/>
    <link:presentationArc order="15" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_PreCommencementTenderOffer_2853f231-a3b2-4df8-9293-5191ff494e3f" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_PreCommencementIssuerTenderOffer_dc35c5c7-89c3-4feb-a194-f06d020f660c" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_PreCommencementIssuerTenderOffer"/>
    <link:presentationArc order="16" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_PreCommencementIssuerTenderOffer_dc35c5c7-89c3-4feb-a194-f06d020f660c" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_96c9a028-cb2d-4b60-af77-1e4b8db3fe93" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_Security12bTitle"/>
    <link:presentationArc order="17" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_Security12bTitle_96c9a028-cb2d-4b60-af77-1e4b8db3fe93" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_71542d06-77ef-4af1-8a14-38a84e5676a7" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_TradingSymbol"/>
    <link:presentationArc order="18" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_TradingSymbol_71542d06-77ef-4af1-8a14-38a84e5676a7" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_9d26ac00-6709-4569-97da-5107cd65a69a" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_SecurityExchangeName"/>
    <link:presentationArc order="19" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_SecurityExchangeName_9d26ac00-6709-4569-97da-5107cd65a69a" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_83227758-672f-464f-8455-a2e6e52223a2" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:presentationArc order="20" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_EntityEmergingGrowthCompany_83227758-672f-464f-8455-a2e6e52223a2" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_d0792195-91ff-4a4c-a52e-47e64d551d25" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_AmendmentFlag"/>
    <link:presentationArc order="21" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_AmendmentFlag_d0792195-91ff-4a4c-a52e-47e64d551d25" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_c53b3e25-afc8-4143-8cd0-0c35f8506b76" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCentralIndexKey"/>
    <link:presentationArc order="22" xlink:arcrole="http://www.xbrl.org/2003/arcrole/parent-child" xlink:from="loc_dei_CoverAbstract_573bbfc4-3cbd-4efd-95c4-43861424aecf" xlink:to="loc_dei_EntityCentralIndexKey_c53b3e25-afc8-4143-8cd0-0c35f8506b76" xlink:type="arc" preferredLabel="http://www.xbrl.org/2003/role/terseLabel"/>
  </link:presentationLink>
</link:linkbase>
